Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Splenocytes from C57BL/6 mice were stained with Rat IgG1 antibodies (left image) or with the corresponding CD73 antibodies as well as with CD3ε antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates. |