Recombinant mouse M-CSF regulates the differentiation, proliferation, and survival of osteoclasts, macrophages, monocytes, and their hematopoietic progenitors. As a potent hematopoietic cytokine, macrophage colony-stimulating factor (M-CSF) is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. This recombinant protein has been optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Mouse M-CSF can be used for a variety of applications, including:
Generation of bone marrow-derived macrophages.
Differentiation of osteoclasts.
Proliferation and apoptosis assays.
Background information
Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
The biological activity of Mouse M-CSF, premium grade, was determined by proliferation assay using M-NFS-60 cells. Activity of Mouse M-CSF, premium grade, (red line) was compared to another commercially available product (black line).
Mouse M-CSF activity assay.
The biological activity of Mouse M-CSF, premium grade, was determined by proliferation assay using M-NFS-60 cells. Activity of Mouse M-CSF, premium grade, (red line) was compared to another commercially available product (black line).
Selected references
Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
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