The Whole Skin Dissociation Kit, human enables the gentle and efficient generation of single-cell suspensions from human skin tissue without separating dermis and epidermis. The kit has been particularly developed for the isolation of fibroblasts from diverse human skin biopsies as well as for phenotyping or enumeration of human skin cell populations by flow cytometry.

Human whole skin can be dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins which maintain the structural integrity of tissues. Dermis is not separated from epidermis. In a first step the human skin is soaked with enzymes which degrade the extracellular matrix. In a second step, single-cells are freed from the extracellular matrix by using the gentleMACS™ Dissociator. Cells should be processed immediately for downstream applications, such as cell culture, cell separation, or cell analysis.