YILIMART - StemMACS Nuclear eGFP mRNA

StemMACS™ Nuclear eGFP mRNA encodes an enhanced green fluorescent protein (eGFP) linked to a nuclear localization signal and is designed for transient transfection.

Applications

Positive control for mRNA transfections
Optimization of mRNA delivery
Localization control

Background information

StemMACS™ Nuclear eGFP mRNA has been designed for transient expression of eGFP in the cell nucleus. The increased nuclear localization of the fluorescent signals facilitates detection by microscopy. StemMACS™ Nuclear eGFP mRNA is recommended as a positive control for mRNA transfections of nuclear factors.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

Nuclear eGFP expression after mRNA transfection:

Human BJ fibroblasts were transfected with 75 ng StemMACS™ Nuclear eGFP mRNA. 24 hours after transfection, eGFP expression (green) and localization in the cell nucleus (colocalization with DAPI) were confirmed by immunofluorescence microscopy.

Untransfected cells	Transfected cells

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StemMACS Nuclear eGFP mRNA

For research use only

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Applications

Background information

Quality description

Figure 1

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