YILIMART - StemMACS Nanog mRNA, human

StemMACS™ Nanog mRNA encodes the human transcription factor Nanog for transient transfection.

Applications

Modulation of pluripotency
Reprogramming

Background information

The homeobox protein Nanog is a key regulator of self-renewal and pluripotency in embryonic stem cells. In combination with other pluripotency factors, Nanog has been used for the generation of induced pluripotent stem cells.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

Nanog expression after mRNA transfection:

Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Nanog mRNA. 24 hours post transfection, Nanog expression (magenta) and nuclear localization (colocalization with DAPI) were analyzed by immunofluorescence microscopy.

Untransfected cells	Nanog-transfected cells

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StemMACS Nanog mRNA, human

For research use only

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Applications

Background information

Detailed procedure

Quality description

Figure 1

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