YILIMART - StemMACS c-Myc mRNA, human

StemMACS™ c-Myc mRNA encodes a stabilized version of the transcription factor c-Myc for transient transfection.

Applications

Modulation of pluripotency
Reprogramming

Background information

c-Myc is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It regulates transcription of a variety of target genes.Together with other transcription factors c-Myc has been used for the generation of induced pluripotent stem cells.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

Increased expression of c-Myc after mRNA transfection:

Human BJ fibroblasts were transfected with 150 ng StemMACS™ c-Myc mRNA. 4 hours after transfection, c-Myc expression (red) was analyzed by immunofluorescence microscopy. Cells transfected with StemMACS™ c-Myc mRNA showed increased levels of c-Myc relative to the untransfected control.

Untransfected cells	c-Myc-transfected cells

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StemMACS c-Myc mRNA, human

For research use only

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Applications

Background information

Detailed procedure

Quality description

Figure 1

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