YILIMART - ODN 2006 1mg

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.

ODN 2006 is a B-class ODN for activation of B cells , induction of Il-6 secretion and activation of NF-kB-signalling pathways.

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells

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. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs

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Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.

Applications

B-class ODN for activation of B cells , induction of Il-6 secretion from human PBMCs, and activation of NF-kB-signalling pathways.

Selected references
1. Hemmi, H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
2. Krieg, A. M. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
3. Bauer, S. et al. (2001) Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. U.S.A. 98: 9237-9242

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ODN 2006 1mg

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Background information

Applications

Selected references

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