LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Mouse LIF can be used for a variety of applications including:
Maintenance of self-renewal and pluripotency in conventional mouse ESC cultures.
Background information
LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.
Mouse LIF activity assay.
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.
Selected references
Rose, T.M. and Bruce, A.G. (1991) Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Natl. Acad. Sci. U.S.A. 88: 8641-8645
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