Detailed separation procedure

• Figure 1

  Untouched B cells were isolated from 30 mL of human EDTA anticoagulated whole blood using the MACSxpress ® Whole Blood B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue ® , CD19-PE, and CD20-FITC and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Non-leukocytes, cell debris, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

  Unseparated fraction

  After separation

• Customer reports and application notes

  MACSxpress

  ^®

  Technology allows isolation of B cells with high purity for sensitive and concordant microarray-based gene expression profiling. Mekes et al. (2013) MACS&more 15(1).