Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Untouched naive B cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ® Whole Blood Naive B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue ® , CD19-PE-Vio ® 770, CD27-APC, and Anti-IgD-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leucocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Unseparated fraction |
After separation |