YILIMART - Human IL-2 IS, research grade 50 μg

Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:

In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4

⁺

T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of CTLL-2 cells (NIBSC 86/504)
premium grade: ≥ 5×

10
⁶
IU/mg

(typical activity: ≥ 9×

10
⁶
IU/mg

)
research grade: ≥ 3×

10
⁶
IU/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 15400 Da.

Mass spectrometry analysis (ESI-MS) of Human IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 15400 Da.
Figure 2
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Figure 2

Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.

SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.
Figure 4
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Figure 4

Human IL-2 IS activity assay.
Activity of Human IL-2 IS, premium grade (red line) was compared to commercially available aldesleukin (black line).

Human IL-2 IS activity assay.
Activity of Human IL-2 IS, premium grade (red line) was compared to commercially available aldesleukin (black line).

Selected references
1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
2. Nguyen, T. L. et al. (2011) Antigen-specific TGF-β-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity. J. Immunol. 187(4): 1745-1753
3. Ng, S. B. et al. (2011) Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma. Blood 118(18): 4919-4929
4. Valignat, M. P. et al. (2013) T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration. Biophys. J. 104(2): 322-331
5. Fuchs, S. et al. (2014)
  
  Patients with T
  ^+/low
  NK
  ⁺
  IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
  
  Eur. J. Immunol. 44(10): 3129-3140
6. Pallett, L. J. et al. (2015) Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells. Nat. Med. 21(6): 591-600
7. Fallarini S. et al. (2012) Invariant NKT cells increase drug-induced osteosarcoma cell death. Br J Pharmakon 167(7): 1533-1549
Certificates

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link
to search for Certificates of Analysis (CoA) by lot number.

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Human IL-2 IS, research grade 50 μg

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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