Background information

Columns

• Figure 1

  Mouse brains (P28) were dissociated using the Neural Tissue Dissociation Kit (P). Flow cytometric analysis shows that the resulting single-cell suspension contains large amounts of myelin membrane fragments and other cellular debris. Only 3% of the signals represent cells (A). Myelin Removal Beads II used with an LS Column and a MidiMACS Separator effectively remove myelin debris (B).

  A:	B:

• Selected references

  1. Pfenninger, C. V. et al. (2007) CD133 is not present on neurogenic astrocytes in the adult subventricular zone, but on embryonic neural stem cells, ependymal cells, and glioblastoma cells. Cancer Res. 67: 5727-5736
  2. Tham, C. S. et al. (2003) Microglial activation state and lysophospholipid acid receptor expression. Int. J. Dev. Neurosci. 21: 431-443

• Brochures and posters

  Neuroscience research

• Scientific posters

  Standardized tissue dissociation and removal of myelin debris optimize isolation of cells from human brain tumor
  Removal of myelin debris after tissue dissociation optimizes immunomagnetic sorting of cells from postnatal brain