YILIMART - Human CD40-Ligand, premium gr. (500μg)

Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human CD40-Ligand can be used for a variety of applications, including:

Stimulation of B cell activation and proliferation.
Stimulation of dendritic cell maturation.
Induction of cytokine production in peripheral blood monocytes and T cells.

Alternative names

TRAP, CD154, TNFSF5

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4

⁺

T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.

Quality description

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of CD19
⁺
B cells
premium grade: ≥ 4×

10
³
U/mg

Figure 1
View details

Figure 1

SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 2
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Figure 2

Mass spectrometry analysis (ESI-MS) of Human CD40-Ligand, premium grade. The major peak corresponds to the calculated molecular mass of 23034 Da. The minor peaks correspond to gluconoylation derivatives as described in Geoghegan, K. F.
et al.
(1999).

Mass spectrometry analysis (ESI-MS) of Human CD40-Ligand, premium grade. The major peak corresponds to the calculated molecular mass of 23034 Da. The minor peaks correspond to gluconoylation derivatives as described in Geoghegan, K. F.
et al.
(1999).
Figure 3
View details

Figure 3

Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 4
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Figure 4

Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
⁺
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
⁺
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).
Figure 5
View details

Figure 5

Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
⁺
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
⁺
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Selected references
1. Spriggs, M.K. et al. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. J. Exp. Med. 176: 1543-1550
Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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Human CD40-Ligand, premium gr. (500μg)

For research use only

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Applications

Alternative names

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

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Figure 3

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Figure 4

Figure 5

Figure 5

Selected references

Certificates

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