YILIMART - Human SCF (25 μg)

Recombinant human SCF (stem cell factor) can stimulate growth and activation of mast cells, as well as expansion of hematopoietic stem cells and myeloid, erythroid, or lymphoid progenitors. The c-Kit receptor transmits the SCF signal, which acts at different levels of embryonic and adult hematopoiesis. For successful use in cell culture, differentiation studies, and functional assays, the recombinant protein human SCF has been optimized.

Applications

Human SCF can be used for a variety of applications, including:

Stimulation of proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures.
In vitro expansion of CD34⁺ hematopoietic progenitor cells.
Differentiation of ES-derived cells towards the hematopoietic lineage.
Mast cell differentiation and maintenance.
Mast cell chemotaxis assays.

Alternative names

c-kit ligand, steel factor, MGF

Background information

SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of TF-1 cells (NIBSC 91/682)
premium grade: ≥ 4×

10
⁵
U/mg

(typical activity: ≥ 9×

10
⁵
U/mg

)
research grade: ≥ 2×

10
⁵
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human SCF, premium grade. The peak corresponds to the calculated molecular mass of 18585 Da.

Mass spectrometry analysis (ESI-MS) of Human SCF, premium grade. The peak corresponds to the calculated molecular mass of 18585 Da.
Figure 2
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Figure 2

Human SCF biological activity.
Activity of Human SCF, premium grade (red bar) was compared to another commercially available product (black bar).

Human SCF biological activity.
Activity of Human SCF, premium grade (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS-PAGE of Human SCF, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human SCF, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 4
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Figure 4

Human SCF activity assay.
The biological activity of Human SCF is determined by proliferation assay using TF-1 cells.

Human SCF activity assay.
The biological activity of Human SCF is determined by proliferation assay using TF-1 cells.

Selected references
1. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
2. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
4. Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
5. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
6. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
7. Brault, J. et al. (2014)
  
  Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
  ex vivo
  models of X
  ⁰
  -linked, AR22
  ⁰
  - and AR47
  ⁰
  - chronic granulomatous diseases.
  
  Biores Open Access 3(6): 311-326
8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
Certificates

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link
to search for Certificates of Analysis (CoA) by lot number.

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Human SCF (25 μg)

For research use only

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Applications

Alternative names

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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