YILIMART - Human M-CSF, premium gr. (100 μg)

Recombinant human M-CSF can regulate the proliferation, differentiation, and survival of monocytes, macrophages, osteoclasts and their hematopoietic progenitors . The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone resorption, atherosclerosis or brain and placental development. M-CSF recombinant protein can be especially used in differentiation studies, cell culture and functional assays.

Applications

Human M-CSF can be used for a variety of applications, including:

Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
Differentiation of macrophages from peripheral blood monocytes.
Differentiation of osteoclasts from CD14⁺ monocytes.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of NFS-60 cells (NIBSC 89/512)
premium grade: ≥ 2×

10
⁷
IU/mg

(typical activity: ≥ 7×

10
⁷
IU/mg

)
research grade: ≥ 1×

10
⁷
IU/mg

Figure 1
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Figure 1

Human M-CSF activity assay.
The biological activity of Human M-CSF is determined by proliferation assay using NFS-60 cells.

Human M-CSF activity assay.
The biological activity of Human M-CSF is determined by proliferation assay using NFS-60 cells.
Figure 2
View details

Figure 2

SDS-PAGE of Human M-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human M-CSF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Selected references
1. Michelet, X. et al. (2015) MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J. Immunol. 194(5): 2079-2088
2. Vogel, S. Z. et al. (2015)
  
  TCAIM decreases T cell priming capacity of dendritic cells by inhibiting TLR-induced Ca
  ²⁺
  influx and IL-2 production.
  
  J. Immunol. 194(7): 3136-3146
3. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
4. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
5. McEwan, W. A. et al. (2013) Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat. Immunol. 14(4): 327-336
6. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
7. Mire-Sluis, A.R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
8. Wang, C. et al. (2010)
  
  Innate immune response to
  Mycobacterium tuberculosis
  Beijing and other genotypes.
  
  PLoS One 5(10): e13594
Certificates

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to search for Certificates of Analysis (CoA) by lot number.

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Human M-CSF, premium gr. (100 μg)

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Selected references

Certificates

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