YILIMART - Human IFN-g1b, premium gr. (25 μg)

IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IFN-γ1b can be used for a variety of applications, including:

Stimulation of monocytes, macrophages, and microglia.
In vitro maturation of Mo-DCs.
Apoptosis and cell growth inhibition assays.
Adhesion and migration assays.

Alternative names

IFN-γ

Background information

IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of HT-29 cells (NIAID Gxg01-902-535)
premium grade: ≥ 2×

10
⁷
IU/mg

(typical activity: ≥ 5×

10
⁷
IU/mg

)
research grade: ≥ 1×

10
⁷
IU/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.

Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.
Figure 2
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Figure 2

Human IFN-γ1b biological activity.
Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).

Human IFN-γ1b biological activity.
Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

Human IFN-γ1b activity assay.
The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).

Human IFN-γ1b activity assay.
The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).
Figure 4
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Figure 4

SDS-PAGE of Human IFN-γ1b, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human IFN-γ1b, premium grade
under reduced (R) and non-reduced (NR) conditions.

Selected references
1. Wong, V.L. et al. (1989) Growth-inhibitory activity of interferon-beta against human colorectal carcinoma cell lines. Int. J. Cancer 43: 526-530
Certificates

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Human IFN-g1b, premium gr. (25 μg)

For research use only

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Applications

Alternative names

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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