YILIMART - Human Flt3-Ligand, premium gr. (10 μg)

Recombinant human Flt3-Ligand can be used to generate and expand hematopoietic stem cells or to differentiate the hematopoietic progenitors. In the myeloid lineage this cytokine is crucial for dendritic cells and in the lymphoid lineage it its involved in B and NK cell differentiation. Human Flt3-Ligand recombinant protein is developed for use in cell culture, differentiation studies, and functional assays.

Applications

Human Flt3-Ligand can be used for a variety of applications, including:

In vitro expansion of CD34⁺ hematopoietic progenitor cells.
Differentiation of ES-derived cells towards the hematopoietic lineage.
In vitro generation of Langerhans cells, dendritic cells, or eosinophils from CD34⁺ cells.

Background information

Fms-related tyrosin kinase 3 ligand (Flt3-Ligand) is a growth factor that regulates early hematopoiesis. Flt3-Ligand belongs to a small family of α-helical cytokines and promotes in synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3 the proliferation and differentiation of primitive hematopoietic stem cells. Early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells is stimulated. Flt3-Ligand is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin. Human Flt3-Ligand is also active on mouse cells.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of OCI-AML5 cells (NIBSC 96/532)
premium grade: ≥ 5×

10
⁵
U/mg
research grade: ≥ 2×

10
⁵
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human Flt3-Ligand, premium grade. The peak corresponds to the calculated molecular mass of 17934 Da.

Mass spectrometry analysis (ESI-MS) of Human Flt3-Ligand, premium grade. The peak corresponds to the calculated molecular mass of 17934 Da.
Figure 2
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Figure 2

Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

Human Flt3-Ligand activity assay.
The biological activity of Human Flt3-Ligand is determined by proliferation assay using OCI-AML5 cells. Activity of Human Flt3-Ligand, premium grade, (red line) was compared to another commercially available product (black line).

Human Flt3-Ligand activity assay.
The biological activity of Human Flt3-Ligand is determined by proliferation assay using OCI-AML5 cells. Activity of Human Flt3-Ligand, premium grade, (red line) was compared to another commercially available product (black line).
Figure 4
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Figure 4

SDS-PAGE of Human Flt3-Ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human Flt3-Ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Selected references
1. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
2. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
4. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
5. Meyer, C. and Drexler, H.G. (1999) FLT3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines. Leuk. Lymphoma 32: 577-581
6. Brault, J. et al. (2014)
  
  Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
  ex vivo
  models of X
  ⁰
  -linked, AR22
  ⁰
  - and AR47
  ⁰
  - chronic granulomatous diseases.
  
  Biores Open Access 3(6): 311-326
Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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Human Flt3-Ligand, premium gr. (10 μg)

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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