G-CSF stands for granulocyte colony stimulating factor. Human G-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Human G-CSF can be used for a variety of applications, including:
Growth promotion and differentiation of cells of the neutrophil lineage.
Induction of colony formation of normal and leukemic bone marrow cells in soft agar cultures.
Background information
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor that affects proliferation and differentiation especially of progenitors of the neutrophil and granulocyte lineages. It is produced mainly by monocytes and macrophages and a variety of other cells like astrocytes, fibroblasts and endothelial cells in response to specific stimulation, for instance by endotoxin, TNF-α and IFN-γ. Furthermore, G-CSF enhances the survival and influences the immunological functions of mature neutrophils. Thus, in addition to its properties as a hematopoietic growth factor G-CSF also acts as a mediator of host defense against infection and inflammatory response.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
The biological activity of Human G-CSF is determined by proliferation assay using NFS-60 cells.
Human G-CSF activity assay.
The biological activity of Human G-CSF is determined by proliferation assay using NFS-60 cells.
Selected references
Shirafuji, N. et al. (1989) A new bioassay for human granulocyte colony-stimulating factor (hG-CSF) using murine myeloblastic NFS-60 cells as targets and estimation of its levels in sera from nomral healthy persons and patients with infectious and hematological disorders. Exp. Hematol. 17: 116-119
Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
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