YILIMART - Neurosphere Dissociation Kit (P)

The Neurosphere Dissociation Kit (P) has been developed for the gentle and efficient generation of single-cell suspensions from cultured neurospheres. In less than an hour, the gentle enzymatic dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further

in vitro

or

in vivo

applications, such as cell culture and expansion, flow cytometry, fluorescence microscopy, and molecular applications, or for cell separations using MACS

^®

Technology.

Background information

Neurospheres are non-adherent spherical clusters of cells formed when neural stem cells are cultured under appropriate conditions. Effective dissociation is important for effective passaging and subsequent downstream applications.

The Neurosphere Dissociation Kit (P) is based on papain. The antigens GLAST (ACSA-1) and PSA-NCAM used to identify some neural populations are sensitive to papain. If preservation of these antigen epitopes is required for downstream applications, it is more suitable to use in this case the Neural Tissue Dissociation Kit (T) (# 130-093-231).

Figure 1

Mouse whole brain was dissociated using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Neural stem cells (NSCs) were isolated via Anti-Prominin-1 MicroBeads and subsequently cultivated in MACS® Neuro Medium supplemented with MACS NeuroBrew-21, penicillin/streptomycin, 2 mM L-glutamine, 20 ng/mL human EGF and FGF-2. A) Primary neurospheres formed by the NSCs after one week in culture. B) Single-cell suspension after dissociation of primary neurospheres using the Neurosphere Dissociation Kit (P). C) Secondary neurospheres formed one week after dissociation.

A:	B:

C:

Brochures and posters

Neuroscience research

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Neurosphere Dissociation Kit (P)

For research use only

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Background information

Figure 1

Brochures and posters

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