Recombinant mouse GM-CSF can regulate growth and differentiation of cells of the myeloid lineage such as DCs, granulocytes, monocytes, and macrophages. As a crucial part of the immune/inflammatory path, the hematopoietic cytokine serves as both an activation signal for mature myeloid cells and survival. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) is developed for use in functional assays, differentiation studies, and cell culture.
Applications
Mouse GM-CSF can be used for a variety of applications, including:
Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
In vitro generation of DCs from bone marrow or maturation of CD11c+ splenocytes.
Generation of antigen-presenting (DC-like) cells in primary brain cell culture.
Background information
GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
The biological activity of Mouse GM-CSF was determined by proliferation assay using mouse FDC-P1 cells. The bioassay was calibrated with the non WHO reference material for mouse GM-CSF (NIBSC code 91/658) provided by the National Institute for Biological Standards and Control.
Mouse GM-CSF activity assay.
The biological activity of Mouse GM-CSF was determined by proliferation assay using mouse FDC-P1 cells. The bioassay was calibrated with the non WHO reference material for mouse GM-CSF (NIBSC code 91/658) provided by the National Institute for Biological Standards and Control.
has biological activity and is neutralized by a specific antiserum.
EMBO J. 4: 2575-2581
Cantini, G. et al. (2012) Immunotherapy against the radial glia marker GLAST effectively triggers specific antitumor effectors without autoimmunity. Oncoimmunology 1(6): 884-893
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