YILIMART - Human IL-7, premium gr. (25 μg)

Recombinant human IL-7 (interleukin 7) stimulates the development of lymphoid progenitors, T, and B cells. The hematopoietic cytokine is crucial for the proliferation of T and B cells, their survival, and homeostasis. Optimized for use in cell culture, functional assays, and differentiation studies.

Applications

Human IL-7 can be used for a variety of applications, including:

In vitro T cell expansion.
In vitro T cell priming.
In vitro differentiation of T cells and iNKT cells.
Investigation of IL-7 mediated signaling pathways.

Background information

IL-7 is a member of the type I cytokine family. The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. It is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells. IL-7 is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of 2E8 cells (NIBSC 90/530)
premium grade: ≥ 5×

10
⁷
U/mg

(typical activity: ≥ 1.5×

10
⁸
U/mg

)
research grade: ≥ 2×

10
⁷
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human IL-7, premium grade. The peak corresponds to the calculated molecular mass of 17382 Da.

Mass spectrometry analysis (ESI-MS) of Human IL-7, premium grade. The peak corresponds to the calculated molecular mass of 17382 Da.
Figure 2
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Figure 2

Human IL-7 biological activity.
Activity of Human IL-7, premium grade (red bar) was compared to another commercially available product (black bar).

Human IL-7 biological activity.
Activity of Human IL-7, premium grade (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS-PAGE of Human IL-7, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human IL-7, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 4
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Figure 4

Human IL-7 activity assay.
The biological activity of Human IL-7, premium grade was determined by proliferation assay using mouse 2E8 cells. The assay was calibrated with the reference standard for human IL-7 (NIBSC 90/530) provided by the National Institute for Biological Standards and Control.

Human IL-7 activity assay.
The biological activity of Human IL-7, premium grade was determined by proliferation assay using mouse 2E8 cells. The assay was calibrated with the reference standard for human IL-7 (NIBSC 90/530) provided by the National Institute for Biological Standards and Control.

Selected references
1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
4. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
5. Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
6. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
Certificates

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to search for Certificates of Analysis (CoA) by lot number.

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Human IL-7, premium gr. (25 μg)

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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