The emission spectra of many fluorochrome conjugates used in flow cytometry overlap with each other, leading to signal detection in more than one fluorescence channel. To correct this overlap, electronic compensation is used.
The MACS® Comp Mouse Igκ Reagent is a dual antibody system selectively targeting human CD52-expressing cells (all leukocytes) as well as the κ light chain of mouse immunoglobulins. The addition of non-labeled cells serves as a negative control.
In contrast to bead-based compensation systems, the cell-based MACS Comp Igκ Reagent system takes into account the autofluorescence of cells during compensation and avoids overcompensation.
