SCF stands for stem cell factor, also termed c-Kit ligand. Mouse SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Mouse SCF may be used for a variety of applications, including:
In vitro culture and expansion of hematopoietic progenitor cells.
Colony formation assays.
Differentiation of ES-derived cells towards the hematopoietic lineage.
Mast cell differentiation and maintenance.
Investigation of receptor signaling.
Alternative names
c-kit ligand, steel factor, MGF
Background information
Stem cell factor (SCF), also known as c-kit ligand, is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms, cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. Recombinant Mouse SCF corresponds to the secreted soluble form.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
The biological activity of Mouse SCF, premium grade, was determined by proliferation assay using TF-1 cells. Activity of Mouse SCF, premium grade, (red line) was compared to another commercially available product (black line).
Mouse SCF activity assay.
The biological activity of Mouse SCF, premium grade, was determined by proliferation assay using TF-1 cells. Activity of Mouse SCF, premium grade, (red line) was compared to another commercially available product (black line).
Selected references
Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
Rodriguez, S. et al. (2011) The SKP2 E3 ligase regulates basal homeostasis and stress-induced regeneration of HSCs. Blood 117(24): 6509-6519
Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
ex vivo
models of X
0
-linked, AR22
0
- and AR47
0
- chronic granulomatous diseases.
Biores Open Access 3(6): 311-326
Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
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