YILIMART - Mouse IL-6 (10 μg)

IL-6 stands for interleukin 6. Mouse IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-6 can be used for a variety of applications, including:

Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
Replacement of feeder cells in the preparation of murine hybridomas.
In vitro differentiation of T_H 17 cells.
Cultivation of bone marrow cells for retroviral transduction.

Background information

Interleukin 6 (IL-6), originally identified as a B cell differentiation factor, is a multifunctional cytokine which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, macrophages, fibroblasts, endothelial cells, and T cells. Disturbed IL-6 production has been associated with pathological processes, including inflammatory autoimmune diseases and cancer.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of 7TD1 cells (NIBSC 93/730)
premium grade: ≥ 4×

10
⁸
U/mg
research grade: ≥ 2×

10
⁸
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Mouse IL-6, premium grade. The peak corresponds to the calculated molecular mass of 21731 Da.

Mass spectrometry analysis (ESI-MS) of Mouse IL-6, premium grade. The peak corresponds to the calculated molecular mass of 21731 Da.
Figure 2
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Figure 2

Mouse IL-6 biological activity.
Activity of Mouse IL-6, premium grade, (red bar) was compared to another commercially available product (black bar).

Mouse IL-6 biological activity.
Activity of Mouse IL-6, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS-PAGE of Mouse IL-6, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Mouse IL-6, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 4
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Figure 4

Mouse IL-6, activity assay.
The biological activity was determined by proliferation assay using mouse 7TD1 cells. Activity of Mouse IL-6, premium grade (red line) was compared to another commercially available product (black line).

Mouse IL-6, activity assay.
The biological activity was determined by proliferation assay using mouse 7TD1 cells. Activity of Mouse IL-6, premium grade (red line) was compared to another commercially available product (black line).