YILIMART - Propidium Iodide Solution

The Propidium Iodide Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis.

Background information

Propidium iodide (PI) is a fluorescent dye that intercalates into double-stranded nucleic acid. It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells.

The fluorescence emission maximum for DNA-bound PI is about 615–620 nm. When excited by a 488 nm laser, PI can therefore be detected in the B2 channel commonly used for PE or in the B3 channel commonly used for PerCP-Vio

^®

700, PE-Cy™5, etc.

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Figure

10
⁶
mouse splenocytes were stained with CD45-VioBlue
^®
for leukocyte detection and with CD90.2-PE for pan T cell detection. Shortly before flow cytometric analysis, 10 μL of Propidium Iodide Solution was added to 1 mL of cell suspension. Cells were analyzed by flow cytometry. Dead cells are positive for PI and thus can be excluded from the analysis. Viable cells belong to the P2 population.

10
⁶
mouse splenocytes were stained with CD45-VioBlue
^®
for leukocyte detection and with CD90.2-PE for pan T cell detection. Shortly before flow cytometric analysis, 10 μL of Propidium Iodide Solution was added to 1 mL of cell suspension. Cells were analyzed by flow cytometry. Dead cells are positive for PI and thus can be excluded from the analysis. Viable cells belong to the P2 population.

Customer reports and application notes

Reliable, hands-free, and high-throughput cell viability assays on the MACSQuant
^®
Flow Cytometer
Special protocols

Protocol for cell viability

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Propidium Iodide Solution

For research use only

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Background information

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Customer reports and application notes

Special protocols

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