IFN-α stands for interferon alpha. Mouse IFN-α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Mouse IFN-α can be used for a variety of applications including:
Invitro investigation of antitumor effects, such as antiproliferative capacities, on tumor cell lines.
Studies on signal transduction and gene expression on cell lines.
In vivo investigation of anti-tumor effects in mouse models of disease.
Studies of transgenic mice harboring the Cre-recombinase transgene driven by the interferon-inducible Mx promoter (Mx-Cre mice).
Background information
Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert multiple functions in the immune system. The most prominent effect of IFN-α is its antiviral activity. Upon viral infections, host cells release IFN-α, which can act in an autocrine or paracrine manner to activate intracellular antiviral defense mechanisms and restrict viral replication. Furthermore, IFN-α affects the generation and function of various dendritic cell populations. Immunomodulatory activity and antitumor effects have been described both
in vivo
and
in vitro
.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
SDS-PAGE of Mouse IFN-alpha, research grade under reduced (R) and non reduced (NR) conditions.
SDS-PAGE of Mouse IFN-alpha, research grade under reduced (R) and non reduced (NR) conditions.
Selected references
Pestka, S. et al. (2004) Interferons, interferon-like cytokines, and their receptors. Immunol. Rev. 202: 8-32
Takaoka, A. and Yanai, H. (2006) Interferon signalling network in innate defence. Cell. Microbiol. 8: 907-922
Darnell, J. E. Jr. et al. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264: 1415-1421
van Boxel-Dezaire, A. H. et al. (2006) Complex modulation of cell type-specific signaling in response to type I interferons. Immunity 25(3): 361-372
Kühn, R. et al. (1995) Inducible gene targeting in mice. Science 269: 1427-1429
Irla, M. et al. (2010) MHC class II-restricted antigen presentation by plasmacytoid dendritic cells inhibits T cell-mediated autoimmunity. J. Exp. Med. 207(9): 1891-1905
Baccala, R. et al. (2012) Anti-IFN-α/β receptor antibody treatment ameliorates disease in lupus-predisposed mice. J. Immunol. 189(12): 5976-5984
Goritzka, M. et al. (2015) Alveolar macrophage-derived type I interferons orchestrate innate immunity to RSV through recruitment of antiviral monocytes. J. Exp. Med. 212(5): 699-714
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