Anti-Biotin-Alkaline Phosphatase has been developed as a secondary antibody for indirect labeling in conjunction with biotinylated primary antibodies. Alkaline phosphatase works with precipitating substrates such as NBT/BCIP as well as soluble substrates such as pNPP to produce a colorimetric reaction.
Technical specifications
Antigen: Biotin
Clone: Bio3-18E7
Isotype: mouse IgG1
Isotype control: Mouse IgG1 – isotype control antibodies
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Methacarn fixed, paraffin-embedded section of a rat lymph node stained with CD45R(B220)-Biotin and Anti-Biotin Alkaline Phosphatase. Color development was achieved using NBT/BCIP as a substrate.
Methacarn fixed, paraffin-embedded section of a rat lymph node stained with CD45R(B220)-Biotin and Anti-Biotin Alkaline Phosphatase. Color development was achieved using NBT/BCIP as a substrate.
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Anti-IgG-APC, human, IS11-3B2.2.3, 100 t