Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Separation of a whole blood sample using the StraightFrom ® Whole Blood CD14 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15-APC, CD14‑PE, as well as CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	After separation

• Selected references

  1. Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
  2. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
  3. Willasch et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis Bone Marrow Transplant. 45: 181-189

• Brochures and posters

  StraightFrom

  ^®

  MicroBeads - Cell isolation directly from blood
  Human myeloid cells from peripheral blood

• Customer reports and application notes

  Willasch

  et al.

  (2012) Enrichment of cell subpopulations by MACS

  ^®

  Technology for PCR-based chimerism analysis. MACS&more 13(1).
  Isolation of monocytes with high purity directly from whole blood for transcriptome analysis in translational research. Foucher et al. (2013) MACS&more 15(1).