Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Separation of a whole blood sample using the StraightFrom ® Whole Blood CD8 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Unlysed cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	After separation

• Selected references

  1. Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
  2. Friedrich, T. C. et al. (2004) Consequences of cytotoxic T-lymphocyte escape: common escape mutations in simian immunodeficiency virus are poorly recognized in naive hosts. J. Virol. 78: 10064-10073
  3. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236

• Brochures and posters

  StraightFrom

  ^®

  MicroBeads - Cell isolation directly from blood