Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Separation of a whole blood sample using the StraightFrom ® Whole Blood CD3 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	After separation

• Selected references

  1. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
  2. Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
  3. Willasch et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis Bone Marrow Transplant. 45: 181-189

• Brochures and posters

  StraightFrom

  ^®

  MicroBeads - Cell isolation directly from blood

• Customer reports and application notes

  Willasch

  et al.

  (2012) Enrichment of cell subpopulations by MACS

  ^®

  Technology for PCR-based chimerism analysis. MACS&more 13(1).