The µMACS and MultiMACS Protein A/G Kits contain colloidal super-paramagnetic MicroBeads, which are conjugated to Protein A/G, respectively. The non-sedimenting Protein A/G MicroBeads ensure rapid reaction kinetics enabling the formation of the labeled immune complex in only 30 minutes. Compared to standard protocols, on-column IPs result in reduced non-specific background binding and less protein dissociation from the complex. Moreover, laborious centrifugation or buffer removal steps can be avoided.

After cell lysis the lysate is incubated with the specific antibody or serum against the target protein. Pre-clearing of the lysate is dispensable. Then the antibody-protein complex is magnetically labeled with µMACS Protein A/G MicroBeads. The sample is loaded onto a MACS Column placed in the magnetic field of the µMACS or thermoMACS Separator. After washing, the magnetically labeled antibody-protein complex and associated molecules are retained on the column. After elution the (co-)immunoprecipitated proteins can be analyzed by SDS-PAGE and Western blot.